Coding

Part:BBa_K3646006:Design

Designed by: Sukanya Chakraborty   Group: iGEM20_IISER_Berhampur   (2020-10-25)


hSTAT2 CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2563
    Illegal BamHI site found at 303
    Illegal BamHI site found at 449
    Illegal BamHI site found at 1602
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
    Illegal NgoMIV site found at 1766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2097


Design Notes

Gene synthesized using Splicing by Overlap Extension. Used as a part of FRaPPe combined CID-FRET reporter to study host-viral PPIs in mammalian HEK293 cells.


Source

Gene sequence of hSTAT2 was sourced from https://www.ncbi.nlm.nih.gov. GenBank Accession : U18671.1

References